Year: 2017 | Month: June | Volume 6 | Issue 1
Studies on Bacteriocin Producing Abilities of Indigenously Isolated Lactic Acid Bacteria Strains
Abstract:
Lactic Acid Bacteria (LAB) known to produce bacteriocins; a small, ribosomally synthesized antimicrobial peptides considered as next generation antibiotics due to the broad spectrum antibiotic nature. Scanty research was reported on the isolation and screening of different LAB species with high bacteriocin yield. With this lacuna the present study aimed to screen various isolated LAB cultures for their bacteriocin yield abilities and subsequently indentifies the elite LAB species representing higher bacteriocin yield. Initially isolated and preserved LAB cultures were sub-cultured and further screened for their bacteriocin yield abilities on CM media as a production media under submerged state fermentation (SmF) conditions. Based on the bacteriocin yield an elite species was identified, and studied morphologically by growing on different media namely Nutrient agar (NA) media, Acetate agarmedia and MRSmedia. Pediococcus pentosaceus strain 2269 obtained from culture collection centre NCIM, Pune, India was used as a standard culture. Microscopic study was performed to screen the visible similarities between the isolate and the standard strain. Further biochemical characterization i.e. Indole, Methyl red, Vogues Proskaeur, Catalase and Carbohydrate fermentation; studies were carried to confirm the culture at Genus level. Finally molecular characterization was done using 16s RNA sequencing followed by phylogenetic analysis (MEGA V-6)to confirm the isolate at species level. Results reported that the culture isolated from the Sanna batter (Sanna 14) was identified as an elite species i.e. Pediococcus pentosaceus with higher bacteriocin yield (0.4mg/l) among the three bacteriocin positives cultures reported in the present study. The 16s RNA sequence of the Pediococcus pentosaceus culture was submitted to NCBI GenBank with an issued accession number MF183113.
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